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Because dh5a, JM109 is a genetically modified strain, and its endogenous nuclease gene has been knocked out by gene, thus greatly improving the stability of plasmid. Wild strain, HB101, Staphylococcus aureus and other strains are rich in nuclease, which leads to a lot of trouble in the extraction of plasmids. When the conventional Plasmid Extraction Kit was used to treat the strain, the plasmid produced was either low in yield or unstable, and it was degraded quickly. Hipure plasma plus kits is designed for wild strains and endo a+, which is rich in nuclease. The kit adopts silica gel column purification technology. The whole operation process does not need to contact with organic matter extraction such as phenol chloroform or alcohol precipitation. The kit also provides high-efficiency alkaline protease, which can hydrolyze and destroy nuclease efficiently and improve the purity of plasmid. The purified plasmid can be used for filing, automatic sequencing, enzyme cutting, PCR and labeling. Hipure plasma pruas micro kit is suitable for extracting 5-20 μ g high copy plasmid DNA from 1-7ml bacterial culture medium.
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