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The HiPure Nucleotide Removal Kit uses silica gel column purification technology, which is suitable for removing oligonucleotides, nucleotides and other impurities smaller than 17 bp from PCR products, enzyme digestion systems, reverse transcription reactions (cDNA), labeling reactions, and enzymatic reaction solutions. Purified DNA or cDNA can be used directly for automated sequencing, ligation, digestion, PCR, labeling, and more.
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| The kit uses glass fiber membrane purification technology, which requires a simple binding-washing-elution step. An appropriate amount of PCR product or reaction solution was added, and then transferred to the purification column for centrifugation or filtration, and the DNA was adsorbed on the glass fiber membrane, and the impurities were removed after two washes, and finally the purified DNA was eluted with a small amount of eluent or water. |
