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HiPure Tissue & Blood DNA kit has been purified by silica gel column, which simplifies the steps of DNA separation from blood, related body fluids, tissues and culture cell samples. No phenol required - Chloroform was extracted without the need to separate the white cells. After the samples were digested directly by protease, they were transferred to the column, and DNA specificity was combined with hipure silica gel column to let the pollution flow through. PCR inhibitors such as bivalent cations and proteins can be completely removed through two effective washing steps. Pure DNA combined with the centrifugal column can be eluted with water or buffer in the kit. HiPure Tissue & Blood DNA kit can be purified from blood, related body fluids, tissues and culture cells to obtain genomic DNA, mitochondrial DNA or virus DNA, and can be directly used in PCR, quantitative PCR, enzyme cutting and imprinting experiments. HiPure Tissue & Blood DNA kit provides a comprehensive manual, including the operational process for purification of DNA from samples from different sources. Source: fresh or frozen whole blood (with anticoagulants such as citrate, EDTA and hepatidic phospholipid); Plasma; Serum; White membrane layer; Bone marrow; Lymphocyte; platelet; Saliva; Secretion, animal tissue and cultured cells.
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High quality DNA - meet a variety of downstream applications, including PCR, quantitative PCR, enzyme digestion, hybridization, etc.
Fast - no white blood cell isolation, no organic matter extraction or ethanol precipitation
Simple - all nucleic acids can be obtained by direct digestion
Extensive - can handle a variety of liquid samples, animal tissues and cultured cells |
